An analytical review on revamping plastic waste management: exploring recycling, biodegradation, and the growing role of biobased plastics.

Globally, 90% of plastics are synthetic, made up of crude oil, natural gas, and coal. Even though plastic is extremely useful in our lives, its excessive use and mismanaged disposal are negatively affecting the ecosystem. The review highlights that the recycling process plays a critical role in controlling the problem of plastic pollution. Although plastic recycling is the most common approach used for managing plastic waste, only 2% of the total plastic waste enters the closed-loop system. However, the review suggests that along with recycling, cost-effective and environmentally friendly plastic approaches can synergistically help to control this increasing problem of plastic waste accumulation. The review further discusses the consequences of plastic pollution on humans and the environment. In particular, the review focuses on biocatalytic and bioengineering tools for the degradation of polyethylene terephthalate (PET), one of the major contributors to plastic waste in landfills and oceans. Moreover, the review presents biobased and biodegradable materials, derived from renewable feedstocks, as an alternative to petroleum-based plastics along with their complete end-of-life options. Overall, this review analyzes the current scenario of the plastic industry, from plastic production to waste generation and management, loopholes and challenges in the current management strategies, and possible solutions like recycling, biodegradation, and biobased plastics.

River rejuvenation in urban India for enhancing living conditions through integrated water resources management.

India, being a developing country, faces big challenges in ensuring water, sanitation, and hygiene (WASH) for all. This case study presents the performance evaluation of a large wastewater management and sanitation-related infrastructure in a metropolitan city in North India. "Dravyavati River Project" is the major sanitation program of the water-stressed Jaipur city based on the concept of river rejuvenation of the long-lost Dravyavati River which flows across the city. The project envisages integrated urban water management such that it aims at the collection and treatment of wastewater (sewage network and treatment plants), safe disposal, ensuring continuous unpolluted flow, geological and ecological integrity to strengthen public health, to reduce the impact of water stress on the total water cycle by promoting groundwater recharge, and improvement in biodiversity. The technical assessment is based on the primary and secondary data collection of field samples and laboratory analysis of influent and effluent samples collected from the five sewage treatment plants (STPs). The results suggest that the project has largely delivered the envisaged environment, public well-being, and ecological and socioeconomic benefits, but there are substantial gaps in the conceived outputs and actual performance. The challenge lies in bridging these gaps and overcoming operational inefficiencies to ensure the sustainability of the Dravyavati River rejuvenation.

Perceiving biobased plastics as an alternative and innovative solution to combat plastic pollution for a circular economy.

Plastic waste from fossil-based sources, including single-use packaging materials, is continuously accumulating in landfills, and leaching into the environment. A 2021 UN Environment Programme (UNEP) report suggests that the plastic pollution is likely to be doubled by 2030, posing a major challenge to the environment and the overall global plastic waste management efforts. The use of biobased plastics such as polyhydroxyalkanoates (PHAs) as a biodegradable substitute for petroleum-based plastics could be a feasible option to combat this issue which may further result in much lower carbon emissions and energy usage in comparison to conventional plastics as additional advantages. Though recent years have seen the use of microbes as biosynthetic machinery for biobased plastics, using various renewable feedstocks, the scaled-up production of such materials is still challenging. The current study outlays applications of biobased plastics, potential microorganisms producing biobased plastics such as Cupriavidus necator, Bacillus sp., Rhodopseudomonas palustris, microalgae, and mixed microbial cultures, and inexpensive and renewable resources as carbon substrates including industrial wastes. This review also provides deep insights into the operational parameters, challenges and mitigation, and future opportunities for maximizing the production of biobased plastic products. Finally, this review emphasizes the concept of biorefinery as a sustainable and innovative solution for biobased plastic production for achieving a circular bioeconomy.

Microbial Biofilms for Environmental Bioremediation of Heavy Metals: a Review.

Heavy metal pollution caused due to various industrial and mining activities poses a serious threat to all forms of life in the environment because of the persistence and toxicity of metal ions. Microbial-mediated bioremediation including microbial biofilms has received significant attention as a sustainable tool for heavy metal removal as it is considered safe, effective, and feasible. The biofilm matrix is dynamic, having microbial cells as major components with constantly changing and evolving microenvironments. This review summarizes the bioremediation potential of bacterial biofilms for different metal ions. The composition and mechanism of biofilm formation along with interspecies communication among biofilm-forming bacteria have been discussed. The interaction of biofilm-associated microbes with heavy metals takes place through a variety of mechanisms. These include biosorption and bioaccumulation in which the microbes interact with the metal ions leading to their conversion from a highly toxic form to a less toxic form. Such interactions are facilitated via the negative charge of the extracellular polymeric substances on the surface of the biofilm with the positive charge of the metal ions and the high cell densities and high concentrations of cell-cell signaling molecules within the biofilm matrix. Furthermore, the impact of the anodic and cathodic redox potentials in a bioelectrochemical system (BES) for the reduction, removal, and recovery of numerous heavy metal species provides an interesting insight into the bacterial biofilm-mediated bioelectroremediation process. The review concludes that biofilm-linked bioremediation is a viable option for the mitigation of heavy metal pollution in water and ecosystem recovery.

Framework to improve biohydrogen generation with estrogen co-metabolism under complete suppression of nitrogen source.

The current work provides insights for improving the hydrogen output while degrading emerging contaminants using Rhodopseudomonas palustris. The changes in the growth rate of a microorganism due to different substrate inputs affects the hydrogen production due to metabolic route changes. The different ratios of glutamate and glycerol as nitrogen and carbon sources along with the presence of ethinylestradiol (EE2) in the photofermenter affected the flux of electrons being directed towards biosynthesis and biohydrogen generation. The combination of glutamate and glycerol in different ratios (Glu:Gly; 0, 0.20 and 0.54) along with estrogen showed no significant difference in the bacteriochlorophyll concentrations. The highest biomass concentration (0.013 h-1) was in ratio of 0.54 while maximum specific hydrogen production (1.9 ± 0.05 ml g-1 biomass h-1) was observed under complete suppression of nitrogen (0; without Glu; non-growing condition) with resultant improved estrogen degradation of about 78% in 168 h by R. palustris strain MDOC01.

Effective salt removal from domestic reverse osmosis reject water in a microbial desalination cell.

Microbial desalination cells (MDC) are evaluated as an environmentally friendly approach for purifying saline water by using power generated by the decomposition of organic materials in the wastewater. The present study is to evaluate the ferrocyanide-redox and biocathode approach in treating simulated saline water and subsequently recovering bio-electricity using actual domestic reverse osmosis reject water. For the desalination of simulated saline water and domestic reverse osmosis reject water, a three-chamber microbial desalination cell with graphite electrodes and anion and cation exchange membranes was constructed. When treating simulated saline water, the biocathode technique achieved a 5% improvement in salt removal and a 4.9% increase in current and power density when compared to the ferrocyanide-redox approach. When biocathode MDC was used to treat domestic reverse osmosis reject water, a maximum current and power density of 3.81 µA/cm2 and 0.337 µW/cm2, respectively, were recorded, as well as COD removal of 83.9% at the desalination chamber and ions reduction for Na, K, and Ca of up to 79%, 76.5%, and 72%, respectively, in a batch operation for 31 days with a stable pH (≈ 7). Thus, the study revealed a microbial desalination cell capable of recovering bioenergy and reducing salt from domestic reverse osmosis reject water with a consistent pH range.

Biodegradation of synthetic estrogen using bioelectrochemical system and degradation pathway analysis through Quadrupole-time-of-flight-mass spectrometry.

Synthetic estrogenic compounds such as 17α-ethinylestradiol (EE2) are significant environmental contaminants. This research studied the biodegradation of EE2 utilizing the EE2 adapted cells isolated from a dairy farm waste site in suspension flask vis-a-vis Bioelectrochemical System (BES) and compared the power output in the BES with and without EE2 as a co-substrate. 78% removal of EE2 was observed in the BES as against 60% removal in suspension flasks. The maximum power density in the BES increased about 53% when EE2 is used as a co-substrate. The EE2 biodegradation studied using HPLC and Q-TOF methods, also proposes a hypothetical pathway for EE2 degradation by the newly isolated strain Rhodopseudomonas palustris MDOC01 and reports the significant metabolites like nicotinic acid and oxoproline being detected during bioelectrochemical treatment process of EE2. Study also suggests that Plasma peroxide treatment of anode material enhanced the overall performance in terms of biodegradation efficiency and power output.

Ultrasonic pre-treatment of Bacillus velezensis for improved electrogenic response in a single chambered microbial fuel cell.

Various microbial strains and techniques are being used to improve power production in microbial fuel cells. Cow dung is a peculiar source of anaerobic and micro-aerophilic organisms that were employed in this study to isolate exo-electrogenic microorganisms. To validate their exo-electrogenic nature, all eight visually distinct bacterial single-cell colonies were tested using the ferrocyanide reduction assay, which resulted in the selection of one bacterial strain AD1-ELB with the ability to reduce ferrocyanide for further biochemical, physiological and electrochemical characterization. The selected strain AD1-ELB was identified as Bacillus velezensis by 16 s rRNA gene sequencing. When used in a single-chambered MFC, the isolated AD1-ELB strain produced a maximum open-circuit voltage of 455 mV with a maximum current density of 51.78 µA/cm2 and maximum power density of 4.33 µW/cm2 on the 16th day. Bacillus velezensis AD1-ELB strain was treated with low-frequency ultrasound (40 kHz) for 1, 2, 3, 4, and 5 min to assess the effect of ultrasonic pre-treatment on an isolated pure culture-based microbial fuel cell. A 3-min exposure to low-frequency ultrasonic therapy resulted in an increase in maximum power of 4.33 µW/cm2 with a current density of 51.78 µA/cm2 in the MFC, which decreases significantly after 4 and 5 min. Thus, the overall power density achieved was 1.89 times greater than in MFCs with untreated strain. These findings support the use of low-frequency ultrasonic stimulation to improve the performance of microbial fuel cell devices and are restricted to the pure, single-cell strain AD1-ELB, with the potential for variation if some other isolated strain is utilized, hence requiring further study to determine its relative variations.

Bioelectrochemical systems for environmental remediation of estrogens: A review and way forward.

Globally estrogenic pollutants are a cause of concern in wastewaters and water bodies because of their high endocrine disrupting activity leading to extremely negative impacts on humans and other organisms even at very low environmental concentrations. Bioremediation of estrogens has been studied extensively and one technology that has emerged with its promising capabilities is Bioelectrochemical Systems (BESs). Several studies in the past have investigated BESs applications for treatment of wastewaters containing toxic recalcitrant pollutants with a primary focus on improvement of performance of these systems for their deployment in real field applications. But the information is scattered and further the improvements are difficult to achieve for standalone BESs. This review critically examines the various existing treatment technologies for the effective estrogen degradation. The major focus of this paper is on the technological advancements for scaling up of these BESs for the real field applications along with their integration with the existing and conventional wastewater treatment systems. A detailed discussion on few selected microbial species having the unusual properties of heterotrophic nitrification and extraordinary stress response ability to toxic compounds and their degradation has been highlighted. Based on the in-depth study and analysis of BESs, microbes and possible benefits of various treatment methods for estrogen removal, we have proposed a sustainable Hybrid BES-centered treatment system for this purpose as a choice for wastewater treatment. We have also identified three pipeline tasks that reflect the vital parts of the life cycle of drugs and integrated treatment unit, as a way forward to foster bioeconomy along with an approach for sustainable wastewater treatment.

Augmenting the biodegradation of recalcitrant ethinylestradiol using Rhodopseudomonas palustris in a hybrid photo-assisted microbial fuel cell with enhanced bio-hydrogen production.

This study presents the biodegradation potential of ethinylestradiol (EE2) in anaerobic environments using exoelectrogenic activity of Rhodopseudomonas palustris. EE2, a basic ingredient in oral contraceptives, is a significant estrogenic micropollutant in various wastewaters and is considered highly recalcitrant. This recalcitrance of EE2 has caused anoxic areas to become repositories for these pollutants. Thus, it is essential to find the microorganisms and suitable methods to degrade this compound. An initial EE2 concentration of 1 mg/L, used in an anaerobic photobioreactor, resulted in 70% EE2 degradation over a period of 16 days with an increase of 63% in hydrogen production when EE2 was used with glycerol as the main carbon source in the culture medium. Furthermore, in the novel setup of hybrid photo-assisted microbial fuel cell (h-PMFC) employed here, EE2 degradation enhanced to 89.82% with a maximum power density of 0.633 ± 0.04 mW/m2. The hybrid MFC employed here could metabolize EE2 and sustained the bio-hydrogen production for 14 days to run the hydrogen fuel cell which otherwise could not be sustained with glycerol only and thus increased the overall power output. The current work highlights the use of R. palustris and the significance of co-metabolism in bioremediation of pollutants and bioenergy generation.

Acetamide hydrolyzing activity of Bacillus megaterium F-8 with bioremediation potential: optimization of production and reaction conditions.

Bacillus megaterium F-8 exhibited an intracellular acetamide hydrolyzing activity (AHA) when cultivated in modified nutrient broth with 3% tryptone, 1.5% yeast extract, and 0.5% sodium chloride, at pH 7.2, 45 °C for 24 h. Maximum AHA was recorded in the culture containing 0.1 M of sodium phosphate buffer, (pH 7.5) at 45 °C for 20 min with 0.2 % of acetonitrile and resting cells of B. megaterium F-8 equivalent to 0.2 ml culture broth. This activity was stable up to 55 °C and was completely inactivated at or above 60 °C. Maximum acyl transferase activity (ATA) was recorded in the reaction medium containing 0.1 M of potassium phosphate buffer, (pH 8.0) at 55 °C for 5 min with 0.85 mM of acetamide as acyl donor and hydroxylamine hydrochloride as acyl acceptor and resting cells of B. megaterium F-8 equivalent to 0.94 mg cells (dry weight basis). This activity was stable up to 60 °C and a rapid decline in enzyme activity was recorded above it. Under the optimized conditions, this organism hydrolyzed various nitriles and amides such as propionitrile, propionamide, caprolactam, acetamide, and acrylamide to corresponding acids. Acyl group transfer capability of this organism was used for the production of acetohydroxamic acid. ATA of B. megaterium F-8 showed broad substrate specificity such as for acetamide followed by propionamide, acrylamide, and lactamide. This amide hydrolyzing and amidotransferase activity of B. megaterium F-8 has potential applications in enzymatic synthesis of hydroxamic acids and bioremediation of nitriles and amides contaminated soil and water system.